What is the principle of most commercially available d-dimer assays?

Study for the ASCP Specialist in Hematology (SH) Test. Prepare with multiple choice questions, each providing hints and explanations. Boost your readiness for the exam!

The principle of most commercially available D-dimer assays is based on using specific antisera to detect the neo-epitopes that are exposed when fibrin is degraded. When a clot forms, fibrin networks are created, and during fibrinolysis, these fibrin molecules are broken down into D-dimers, which are small protein fragments.

The antibodies in the assay are designed to recognize and bind specifically to these D-dimer fragments, allowing for their quantification. This specificity is crucial because it enhances the assay’s ability to differentiate between normal fibrinogen and the fragments produced specifically during fibrinolysis, leading to a more accurate assessment of clot formation and breakdown in the body.

The other answer options do not accurately reflect the principle of D-dimer assays. Measuring the time it takes a patient's clot to lyse pertains to different aspects of hemostasis, while measuring plasminogen levels does not directly relate to D-dimer detection. Adding thrombin to patient plasma would influence coagulation but is not a method for quantifying D-dimers. Thus, the use of specific antisera to detect these neo-epitopes is the correct understanding of how the assays work.

Subscribe

Get the latest from Examzify

You can unsubscribe at any time. Read our privacy policy